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nl557 conjugated tra 1 60  (R&D Systems)


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    R&D Systems nl557 conjugated tra 1 60
    Nl557 Conjugated Tra 1 60, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nl557+conjugated+tra+1+60/Human+GloLIVE+TRA-1-60(R)+NorthernLights%E2%84%A2+NL557-conjugated+Antibody/bio_rxiv__2024__12__28__630627-202-10-12
    Average 90 stars, based on 6 article reviews
    nl557 conjugated tra 1 60 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Marker:

    Article Title: Bovine Formative Embryonic Stem Cell Plasticity in Embryonic and Extraembryonic Differentiation
    Article Snippet: Alexa Fluor 488, 594 or 647 conjugated goat anti-rabbit or goat anti-mouse secondary antibody (Cell Signaling Technology) were used in 1:1000 dilution. .. For cell surface marker staining, the cells were stained with NL557-conjugated TRA-1-60 (R&D Systems, NLLC4770R), SSEA-3 (R&D Systems, MAB1434) and Alexa Fluor 594 conjugated SSEA-4 (BioLegend, 330414) according to the manufacturer’s protocols. .. Bovine XIST probes with the Quasar 570 Dye and HUWE1 probes with the Quasar 670 Dye were purchased from Biosearch Technologies (SMF-1063-5 and SMF-1065-5).

    Staining:

    Article Title: Bovine Formative Embryonic Stem Cell Plasticity in Embryonic and Extraembryonic Differentiation
    Article Snippet: Alexa Fluor 488, 594 or 647 conjugated goat anti-rabbit or goat anti-mouse secondary antibody (Cell Signaling Technology) were used in 1:1000 dilution. .. For cell surface marker staining, the cells were stained with NL557-conjugated TRA-1-60 (R&D Systems, NLLC4770R), SSEA-3 (R&D Systems, MAB1434) and Alexa Fluor 594 conjugated SSEA-4 (BioLegend, 330414) according to the manufacturer’s protocols. .. Bovine XIST probes with the Quasar 570 Dye and HUWE1 probes with the Quasar 670 Dye were purchased from Biosearch Technologies (SMF-1063-5 and SMF-1065-5).



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    Characterization of primed-like bovine iPSCs. ( A ): Flat colony morphology of 4-1 in KT medium. Bar = 250 µm. ( B ): Karyotype for O4 and 4-1 biPSC lines at passage 36 and 42, respectively. ( C ): qRT-PCR for endogenous expression of pluripotent genes in four lines of biPSCs at early passages (4-1 at P17, 4-6 at P17, 3-2 at P10) and later passages (4-1 at P43, 4-6 at P25, 3-2 at P29, O4 at P40). Bar = mean ± sd, n = 4. One way–ANOVA with Tukey’s post hoc multiple comparison test was used for data analysis. ( D ): Representative immunostaining images of biPSCs in KT medium for OCT4, NANOG, SOX2, SSEA3, SSEA4, <t>TRA-1-60.</t> Bar = 120 µm. ( E ): EBs formed from 4-1_KT, bar = 625 µm. ( F ): Immunostaining of differentiated cells for the three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs. bar = 120 µm.
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    Characterization of primed-like bovine iPSCs. ( A ): Flat colony morphology of 4-1 in KT medium. Bar = 250 µm. ( B ): Karyotype for O4 and 4-1 biPSC lines at passage 36 and 42, respectively. ( C ): qRT-PCR for endogenous expression of pluripotent genes in four lines of biPSCs at early passages (4-1 at P17, 4-6 at P17, 3-2 at P10) and later passages (4-1 at P43, 4-6 at P25, 3-2 at P29, O4 at P40). Bar = mean ± sd, n = 4. One way–ANOVA with Tukey’s post hoc multiple comparison test was used for data analysis. ( D ): Representative immunostaining images of biPSCs in KT medium for OCT4, NANOG, SOX2, SSEA3, SSEA4, <t>TRA-1-60.</t> Bar = 120 µm. ( E ): EBs formed from 4-1_KT, bar = 625 µm. ( F ): Immunostaining of differentiated cells for the three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs. bar = 120 µm.
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    Characterization of primed-like bovine iPSCs. ( A ): Flat colony morphology of 4-1 in KT medium. Bar = 250 µm. ( B ): Karyotype for O4 and 4-1 biPSC lines at passage 36 and 42, respectively. ( C ): qRT-PCR for endogenous expression of pluripotent genes in four lines of biPSCs at early passages (4-1 at P17, 4-6 at P17, 3-2 at P10) and later passages (4-1 at P43, 4-6 at P25, 3-2 at P29, O4 at P40). Bar = mean ± sd, n = 4. One way–ANOVA with Tukey’s post hoc multiple comparison test was used for data analysis. ( D ): Representative immunostaining images of biPSCs in KT medium for OCT4, NANOG, SOX2, SSEA3, SSEA4, <t>TRA-1-60.</t> Bar = 120 µm. ( E ): EBs formed from 4-1_KT, bar = 625 µm. ( F ): Immunostaining of differentiated cells for the three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs. bar = 120 µm.
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    Characterization of primed-like bovine iPSCs. ( A ): Flat colony morphology of 4-1 in KT medium. Bar = 250 µm. ( B ): Karyotype for O4 and 4-1 biPSC lines at passage 36 and 42, respectively. ( C ): qRT-PCR for endogenous expression of pluripotent genes in four lines of biPSCs at early passages (4-1 at P17, 4-6 at P17, 3-2 at P10) and later passages (4-1 at P43, 4-6 at P25, 3-2 at P29, O4 at P40). Bar = mean ± sd, n = 4. One way–ANOVA with Tukey’s post hoc multiple comparison test was used for data analysis. ( D ): Representative immunostaining images of biPSCs in KT medium for OCT4, NANOG, SOX2, SSEA3, SSEA4, TRA-1-60. Bar = 120 µm. ( E ): EBs formed from 4-1_KT, bar = 625 µm. ( F ): Immunostaining of differentiated cells for the three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs. bar = 120 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Establishment of Bovine-Induced Pluripotent Stem Cells

    doi: 10.3390/ijms221910489

    Figure Lengend Snippet: Characterization of primed-like bovine iPSCs. ( A ): Flat colony morphology of 4-1 in KT medium. Bar = 250 µm. ( B ): Karyotype for O4 and 4-1 biPSC lines at passage 36 and 42, respectively. ( C ): qRT-PCR for endogenous expression of pluripotent genes in four lines of biPSCs at early passages (4-1 at P17, 4-6 at P17, 3-2 at P10) and later passages (4-1 at P43, 4-6 at P25, 3-2 at P29, O4 at P40). Bar = mean ± sd, n = 4. One way–ANOVA with Tukey’s post hoc multiple comparison test was used for data analysis. ( D ): Representative immunostaining images of biPSCs in KT medium for OCT4, NANOG, SOX2, SSEA3, SSEA4, TRA-1-60. Bar = 120 µm. ( E ): EBs formed from 4-1_KT, bar = 625 µm. ( F ): Immunostaining of differentiated cells for the three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs. bar = 120 µm.

    Article Snippet: For cell surface marker staining, the cells in different reprogramming conditions were stained with NL557-conjugated TRA-1-60 (1:100), SSEA-3 (1:50; R&D Systems, Minneapolis, MN, USA) and Alexa Fluor 594 conjugated SSEA-4 (1:100; BioLegend, San Diego, CA, USA) according to the manufacturer’s protocol.

    Techniques: Quantitative RT-PCR, Expressing, Comparison, Immunostaining, Passaging

    Characterization of naïve-like bovine iPSCs. ( A ): Flat, monolayered primed-like 4-1 biPSC colonies cultured in KT medium and passaged with collagenase (left), deteriorated colony morphology upon trypsinization (middle), and dome-shaped, naïve-like biPSCs cultured in TiF medium and passaged with trypsin (right), bar = 250 µm. ( B ): Cell proliferation difference between naïve and primed-like biPSCs. Mean ± sd, n = 3. Student’s t-test was used for data comparison. ( C ): immunostaining of naïve-like 4-1 biPSCs in TiF medium with OCT4, NANOG, SOX2, SSEA3, SSEA4, TRA-1-60 antibodies. Bar = 120 µm. ( D ): Immunostaining of EBs derived from naïve-like biPSCs for three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs, bar = 120 µm. ( E ): Bisulfite sequencing results of bovine OCT4 distal enhancer (Left Panel) and proximal promoter (Right Panel) genomic regions. Open and closed circles represent unmethylated and methylated CpGs, respectively. The percentage of methylated CpG is shown at the bottom of each sample. ( F ): Bisulfite sequencing results of bovine NANOG promoter genomic region. Open and closed circles represent unmethylated and methylated CpGs, respectively. The percentage of methylated CpG is shown at the bottom of each sample.

    Journal: International Journal of Molecular Sciences

    Article Title: Establishment of Bovine-Induced Pluripotent Stem Cells

    doi: 10.3390/ijms221910489

    Figure Lengend Snippet: Characterization of naïve-like bovine iPSCs. ( A ): Flat, monolayered primed-like 4-1 biPSC colonies cultured in KT medium and passaged with collagenase (left), deteriorated colony morphology upon trypsinization (middle), and dome-shaped, naïve-like biPSCs cultured in TiF medium and passaged with trypsin (right), bar = 250 µm. ( B ): Cell proliferation difference between naïve and primed-like biPSCs. Mean ± sd, n = 3. Student’s t-test was used for data comparison. ( C ): immunostaining of naïve-like 4-1 biPSCs in TiF medium with OCT4, NANOG, SOX2, SSEA3, SSEA4, TRA-1-60 antibodies. Bar = 120 µm. ( D ): Immunostaining of EBs derived from naïve-like biPSCs for three-germ layer markers (AFP for endoderm, SMA for mesoderm, and TUJ1 for ectoderm) after passaging of the EBs, bar = 120 µm. ( E ): Bisulfite sequencing results of bovine OCT4 distal enhancer (Left Panel) and proximal promoter (Right Panel) genomic regions. Open and closed circles represent unmethylated and methylated CpGs, respectively. The percentage of methylated CpG is shown at the bottom of each sample. ( F ): Bisulfite sequencing results of bovine NANOG promoter genomic region. Open and closed circles represent unmethylated and methylated CpGs, respectively. The percentage of methylated CpG is shown at the bottom of each sample.

    Article Snippet: For cell surface marker staining, the cells in different reprogramming conditions were stained with NL557-conjugated TRA-1-60 (1:100), SSEA-3 (1:50; R&D Systems, Minneapolis, MN, USA) and Alexa Fluor 594 conjugated SSEA-4 (1:100; BioLegend, San Diego, CA, USA) according to the manufacturer’s protocol.

    Techniques: Cell Culture, Comparison, Immunostaining, Derivative Assay, Passaging, Methylation Sequencing, Methylation